Lloyd Rossouw obtained his BA degree, majoring in Archaeology and Cultural Anthropology, and went on to receive training in southern African archaeology at Honours level at the University of Stellenbosch Archaeology Department. He received specialized training in faunal osteology and Quaternary palaeontology for his MSc (cum laude) at the Bernard Price Institute for Palaeontological Research (BPI) at the Univesity of the Witwatersrand.
He also received instruction in human anatomy at Duke University Medical School in Durham, North Carolina, USA. For his PhD degree, obtained in 2009 at the University of the Free State, he developed an archaeobotanical method that can be applied to fossil grass phytoliths to estimate past environmental conditions.
He is a member of the Association for South African Professional Archaeologists (ASAPA) as well as a member of its Cultural Resource Management (CRM) section, with accreditation in the following areas of professional archaeological specialization: Principal Investigator, Specialist Analysis for archaeobotany and archaeozoology and Field Supervisor for Stone Age archaeology.